FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_Emily_2_Mar_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_Emily_2_Mar_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8934785
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT38250.027.6981161
AACCGTG39950.026.313222
ACCGTGT54650.018.704743
CCGTGTT59550.017.530874
CGTGTTC64400.016.773485
CGGGAAT25850.015.7201371
TTCACTA67350.015.5010039
ATTAGGC37150.014.4409465
GTTCACT77350.013.5907248
TATTAGG41350.012.9741524
CTTTATT142600.012.2129491
TGTTCAC92650.011.7376387
CGCGAAT6303.9196668E-511.5182971
CGGAAAT27800.011.2241251
GTGTTCA102950.011.1263696
CGAATCG19700.011.0402213
AACTCAC75350.010.6797855
ACAACCG30150.010.580062
CGGGTAT13753.6379788E-1110.5549491
CGTATCA88300.010.509256145
Produced by FastQC (version 0.11.5)