FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_Emily_2_Mar_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_Emily_2_Mar_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11584586
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG296310.25577953325220254No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC272220.23498465978844646No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC222830.19235042149974113No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA209760.18106818836685232No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG197760.17070959635501864No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC191750.16552166818909195No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC190720.16463255570807622No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC165750.14307805216345237No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA164830.1422838934425451No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA149580.1291198494275065No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA141510.12215369629954838No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA140260.12107467629831571No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG138510.11956404829658997No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC138270.1193568764563533No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA135570.11702619325369072No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG124340.1073322775626164No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT123030.10620146460132456No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT122280.10555405260058495No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT120210.10376719547854364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT73250.062.8083571
AACCGTG78400.058.4413832
ACCGTGT103400.043.9608843
CCGTGTT113250.039.9453124
CGTGTTC126700.036.1054045
ATTAGGC102050.030.4764065
TATTAGG108400.028.9586334
TGTTCAC164050.028.7697097
TTCACTA161150.028.3426829
GTTCACT166150.027.8388338
GTGTTCA172950.027.162766
TTAGGCA119050.026.2462526
AGACTCA120500.025.5694454
TTATTAG125350.025.5633283
ACAGACT116000.024.3114622
ACAACCG43700.024.2209342
CTTTATT327850.022.709521
TCACTAG153850.022.4771043
CTCAGAA215550.022.3876971
CACTAGC153400.022.3067424
Produced by FastQC (version 0.11.5)