FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_Emily_2_Mar_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_Emily_2_Mar_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17298510
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG376570.21768926919139278No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC338880.19590126548471518No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA296900.1716332793980522No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG270600.15642965781445917No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC270120.15615217726844682No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC222230.12846771195900686No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA197970.1144433826959663No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC196890.11381905146743852No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA186310.10770291776574976No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA184830.10684735274887837No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA177820.10279498060815644No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC174110.10065028722126934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT101550.059.4571881
AACCGTG112700.053.198632
ACCGTGT148200.040.308733
CCGTGTT162000.036.696024
CGTGTTC183500.032.594035
ATTAGGC145700.029.3570615
TATTAGG160500.026.5144864
TGTTCAC235050.025.9087647
TTCACTA230850.025.8776599
GTTCACT236100.025.517188
GTGTTCA249650.024.828746
TTAGGCA178650.023.9424786
TTATTAG187700.022.9812053
ACAACCG66100.022.1548482
TCACTAG213350.021.9512863
AGACTCA170150.021.7299334
CACTAGC214300.021.6848284
AACTCAC229500.021.4490095
ACTAGCA225250.020.759415
CTTTATT473200.020.5504341
Produced by FastQC (version 0.11.5)