FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_Emily_2_Mar_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_Emily_2_Mar_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9079495
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT33300.033.125141
AACCGTG38100.029.3034422
ACCGTGT53000.021.4756683
CACTAGC62150.021.4634534
TCACTAG64300.021.0840243
TTCACTA73350.020.4594122
ACTAGCA67550.019.7476485
CCGTGTT58700.019.1432884
TAGCAAC69250.018.8444217
CTAGCAA73800.017.9770166
GTTCACT81150.017.885451
CGTGTTC66350.017.5917025
AACTCAC81950.016.8084455
ACTCACC88650.015.7834346
AGGTTGA80000.014.8619834
ATTAGGC41700.014.7776625
TGTTCAC91400.013.7223977
GTTGAGG91600.013.3756266
GGTTGAG91550.013.2245535
GAACTCA116100.013.1756844
Produced by FastQC (version 0.11.5)