FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_Emily_2_Mar_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_Emily_2_Mar_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17732085
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233950.1319359793278681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT55950.018.028971
AACCGTG66700.014.6735542
CTTTATT264650.012.06526851
CGCGAAT10404.292815E-1011.862381
ATTAGGC63500.011.7595555
TCACTAG89550.011.17225553
CGGGAAT54800.010.9914181
ACCGTGT95400.010.8671343
CGTATCA175750.010.848861145
CGCCGTA223350.010.711535145
CACTAGC87800.010.651794
CGCGATT8951.1227658E-610.5408721
TTCACTA111850.010.5004142
CGGGCAT77550.010.4807521
TATTAGG75700.010.1516684
GTATCAT207300.010.141973145
CCGTATC194800.010.12287145
CGGGGAT90750.010.0758141
CCGTGTT103050.09.9196994
CGGAAAT53250.09.8122621
Produced by FastQC (version 0.11.5)