FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_Emily_2_Mar_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_Emily_2_Mar_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13437991
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180330.1341941663750184No Hit
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG144550.10756816253262858No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT62000.036.4016271
AACCGTG69300.032.2212752
ACCGTGT91750.024.4951933
CCGTGTT99500.022.3686894
CGTGTTC114250.019.7981055
TTCACTA123100.018.1393269
GTTCACT137450.016.7203228
ACAACCG48400.016.0274222
AGACTCA131650.015.80474
TGTTCAC155750.015.5470687
CTTTATT264850.013.9740491
CACTAGC98600.013.9701784
GTGTTCA170450.013.9508956
ATTAGGC72950.013.8138495
TCACTAG102950.013.5207293
AACTCAC126850.013.0879085
ACTCACC131000.012.3412416
TAGCAAC112150.012.1531447
CTAGCAA111600.012.147946
ACTAGCA112200.012.14759355
Produced by FastQC (version 0.11.5)