FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_Emily_2_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_Emily_2_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8378330
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT100850.09.560855145
GCGTCGG20101.8189894E-129.017058145
TGGTCGC131250.08.892984145
GTGGTCG143200.08.454627145
CTCGGTG192550.08.132633145
GCCGTAT93350.08.076812145
TCGGTGG182200.08.037555145
CCGTATC88550.07.9415293145
CGCCGTA94800.07.8768005145
CCGGATC12507.759809E-57.5397043145
TCTCGGT199350.07.527921145
GGTCGCC123600.07.3318324145
GATCTCG242950.07.2512074145
GCGTCGA4201.3345925E-87.249785140-144
CGTATCA86500.07.2078786140-144
GTCGCCG114800.07.1991954145
CGGTGGT169750.07.174976145
GGTGGTC184000.07.092113145
ATCGGAC14350.07.0728936145
GTGTAGA332450.07.0436406145
Produced by FastQC (version 0.11.5)