FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_Emily_2_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_Emily_2_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10552708
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG5107.080718E-512.793655145
GCGTCGG37050.012.718839145
GTCGCCG251200.09.697102145
GGTCGCC271600.09.048826145
GCCGTAG11252.1393826E-69.021896145
TCGCCGT218500.08.991666145
GCCGTAT194750.08.710855145
CGTATCA177500.08.536311145
CGCCGTA203750.08.503987145
ATCGGAC22601.8189894E-128.340406145
TGGTCGC285950.08.138366145
CTCGGTG398950.08.068389145
CGTCGAG8150.00526113768.005846145
GTGGTCG310050.07.973426145
TCTCGGT415200.07.909758145
CGGTGGT359600.07.781977145
CCGTATC183700.07.537833145
GATCTCG491400.07.4798884145
GAGCGAC15903.6721813E-67.295335145
CGTCGGG32150.07.215914145