FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15714813
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG43700.056.825732
ACCGTGT50850.046.39923
CCGTGTT52500.043.2913174
GTTCACT82450.027.6441738
ATTAGGC49300.022.2457075
TATTAGG47200.022.1653984
TTCACTA121250.019.7498389
CAACCGT139850.017.9118541
TTAGGCA61400.017.6267596
CTTTATT139250.017.3150921
TCACTAG105100.017.3002173
TTATTAG61100.017.0049343
CTAGCAA115050.016.9327186
CGTGTTC136050.016.8116465
CACTAGC113800.016.8016974
TAGCAAC127100.015.9465957
GGTTGAG60300.015.6748555
ACTAGCA125850.015.5369445
GGAGGTT62750.014.8403432
CTCAGAA183400.013.619161
Produced by FastQC (version 0.11.5)