FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n01_Emily_2_Mar_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n01_Emily_2_Mar_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20046944
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC272320.13584115364416643No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC258400.1288974519008982No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA215850.1076722716439972No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC214060.10677936746867753TruSeq Adapter, Index 11 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT80950.037.1863751
AACCGTG88850.033.7982672
ACCGTGT127900.023.8644083
CACTAGC151400.023.2243824
TCACTAG157300.022.5382023
ACTAGCA163750.021.8712675
TTCACTA172250.021.3080042
CCGTGTT144400.021.036584
TAGCAAC173000.020.4923177
CTAGCAA174100.020.4044846
GTTCACT192900.019.8542861
CGTGTTC154250.019.6932375
AACTCAC227150.018.8626655
ATTAGGC112000.018.577715
ACTCACC237000.017.9563486
TATTAGG118850.017.262974
AGACTCA190950.015.5285884
TGTTCAC206200.015.36459357
AGGTTGA196900.014.948884
GAACTCA312500.014.6620739
Produced by FastQC (version 0.11.5)