FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n01_Emily_2_Mar_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n01_Emily_2_Mar_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23859083
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT77700.016.5245721
AACCGTG90400.014.0426032
CGAATCG69800.011.1139573
GGCGAAT99700.010.4044441
CGGGAAT75300.010.3078191
CGGGGAT118750.010.0792521
ACCGTGT128850.010.0155773
GCGAATC92300.09.7453622
GAATCGT94000.09.6407274
GGGGAAT180700.09.5542551
GCCGAAT60250.09.5114781
CGGAAAT76350.09.5009921
ACGATCA318850.09.459044145
CCGTGTT145300.09.230684
GTCACGA377800.09.153703145
CGTATGC102700.09.106671145
AATCGTA88750.09.06738955
CGCGAAT14601.1819793E-88.9432971
TTCACTA146900.08.93273459
TCGTATG94200.08.927862145
Produced by FastQC (version 0.11.5)