FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n01_Emily_2_Mar_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n01_Emily_2_Mar_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8934785
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT36800.028.7782861
AACCGTG38300.027.2722662
ACCGTGT53950.019.2170073
CCGTGTT59900.018.0338484
TTCACTA66050.017.2327989
CGTGTTC67200.016.3984685
GTTCACT77050.014.6784748
ATTAGGC37550.014.6734635
TATTAGG39850.014.55427554
CTTTATT143800.013.4682851
TGTTCAC95900.012.2468787
ACAACCG30000.012.0894412
AGACTCA83800.011.6793514
TTATTAG55500.011.4955783
TCACTAG53800.011.3197833
GTGTTCA105550.011.0585116
CGGGAAT25800.010.9649041
CGACGAT9502.10508E-710.68967151
CACTAGC55550.010.4408264
CGGCAAT21550.010.4345651
Produced by FastQC (version 0.11.5)