FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n01_Emily_2_Mar_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n01_Emily_2_Mar_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9079495
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT34150.039.7241331
AACCGTG39200.035.16162
ACCGTGT55150.024.7145483
CCGTGTT64000.021.2964024
CGTGTTC70150.020.1527945
CACTAGC61200.019.6645974
TTCACTA71000.019.4009789
TCACTAG64000.018.918073
TAGCAAC67050.018.1651467
ACTAGCA69350.017.5626955
GTTCACT84450.016.665011
CTAGCAA74050.016.3500776
ACTCACC89200.015.7675866
ATTAGGC45650.015.5637295
TGTTCAC93600.015.4911087
AACTCAC88550.015.4739655
GCGTAAT5857.596282E-814.8808871
GTGTTCA102300.014.2445526
GGCGAAT21100.014.09629251
TATTAGG51450.014.0910384
Produced by FastQC (version 0.11.5)