FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n01_Emily_2_Mar_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n01_Emily_2_Mar_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21141774
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC644210.3047095291057411No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC625960.29607733012376347No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA522630.24720252898361322No Hit
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG465910.22037412754483138No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA395440.18704201454428562No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC363850.17210003285438583No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA361700.17108308886472817No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT348880.16501926470314177No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC332450.15724792063333948No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC293340.1387489999656604No Hit
GGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCG290560.13743406773717284No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA255110.12066631683793423No Hit
TGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGAC248870.11771481428190464No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG243030.11495251060767181No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT236560.11189221869460907No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA226550.10715751667764493No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT223890.10589934411369642No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC222980.10546891665760877No Hit
TCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCC221830.10492496987244307No Hit
GGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTCTCTCCAGAG212500.10051190595453342No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG212110.10032743704478159No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG211550.1000625586102661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT113250.065.844991
AACCGTG127150.058.7038842
ACCGTGT177250.041.8435133
CCGTGTT194450.038.1041644
CGTGTTC214800.034.6967125
CACTAGC274700.029.163064
TCACTAG274200.028.7144873
ACAGACT210150.028.5113962
ACTAGCA284550.028.1025945
TGTTCAC271350.028.0001877
TTCACTA286150.027.6059062
AGACTCA230700.027.3551752
GTTCACT296050.026.976781
GTGTTCA291350.026.2025056
CTAGCAA306600.026.057876
TAGCAAC311450.025.7219227
CTCAGAA397650.024.7358321
ATTAGGC148400.023.1565535
AACTCAC388300.022.983715
AGGTTGA245150.022.712184
Produced by FastQC (version 0.11.5)