FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n01_Emily_2_Mar_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n01_Emily_2_Mar_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13062414
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA345960.2648515044768907No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC332280.25437870825407927No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC259220.1984472395378067No Hit
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG228630.17502890353957545No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA201130.15397613335483012No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG180490.13817507238707943No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA175450.1343166737786752No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT174600.1336659517911467No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA169360.12965444212685343No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC162010.1240276108229306No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC161780.12385153310865817No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA145340.1112658043145777No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG143200.10962751601656477No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT140130.10727726130866776No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA137310.10511839542063206No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA134170.10271455184317387No Hit
GGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCG132430.10138248565693907No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA131770.10087721917250517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT67400.056.5006981
AACCGTG74050.051.8185232
ACCGTGT100600.038.412933
CCGTGTT112800.034.1926274
TCACTAG166500.030.7424643
CACTAGC168100.030.6642134
TTCACTA174850.029.4541932
CGTGTTC133050.029.2610135
ACTAGCA178850.028.9021745
GTTCACT183200.028.5472451
ATTAGGC106450.028.4682355
TATTAGG112900.026.713414
CTAGCAA194250.026.6108326
AACTCAC193100.026.4689545
TAGCAAC196150.026.2421847
ACTCACC198750.025.4976396
TTAGGCA124850.024.4468826
TTATTAG127300.023.7495773
TGTTCAC171150.023.1707637
GAACTCA234500.022.5688764
Produced by FastQC (version 0.11.5)