FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n01_Emily_2_Mar_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n01_Emily_2_Mar_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17732085
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT62500.016.7124421
AACCGTG68450.015.2597182
ATTAGGC63800.012.4997235
CTTTATT264300.011.9933831
GATTGAG124150.011.2119955
CGTATGC234050.010.996803145
CGGGTAT27300.010.8937821
TATGCCG207600.010.826327145
ACCGTGT97200.010.7408663
CGGGAAT54050.010.6020251
AACTCAC128700.010.5902865
CTCGTAT227650.010.573462145
CGGGCAT76450.010.5318091
TATTAGG76650.010.4987894
TATCTCG279700.010.109249145
CGGCAAT45600.010.021511
CCGTGTT103550.09.9418364
TCGTATG199450.09.923739145
CTTATCG123000.09.902638145
GGCGAAT63350.09.8471211
Produced by FastQC (version 0.11.5)