FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n01_Emily_2_Mar_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n01_Emily_2_Mar_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13437991
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG151220.11253170209743406No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT63750.040.1663671
AACCGTG68700.037.061112
ACCGTGT91500.028.287553
CCGTGTT98900.025.6567824
CGTGTTC114050.022.5664635
TTCACTA121000.021.150469
GTTCACT136900.018.6939778
TGTTCAC159850.016.7810657
ATTAGGC74900.016.7453695
GTGTTCA179650.015.657956
AGACTCA131900.015.6100394
ACAACCG48100.015.5331742
TATTAGG85950.014.8455824
CTTTATT275900.013.6979021
ACAGACT147200.012.6153762
CGGGAAT35900.012.5275251
TTAGGCA100250.012.51100356
TTATTAG108750.012.2669553
CAGACTC180800.012.0301493
TCACTAG103700.012.0253543
Produced by FastQC (version 0.11.5)