FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n01_Emily_2_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n01_Emily_2_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8378330
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT6001.6467693E-613.29186145
CTCGTAT86750.09.360366145
ACGATCG37850.09.194321145
ATCGCGT26650.08.705567145
ATGCCGT61150.08.417947145
TCGTATG70800.08.294611145
TCTCGTA94750.07.8813806145
CATCACG248800.07.7221775145
ATCTCGT114750.07.581809145
CGACGCT3308.006009E-77.469537715-19
CCGTCGT11650.07.46792145
TATGCCG63300.07.444815145
CACGATC210350.07.4103756145
ATCACGA254400.07.3811994145
TCACGAT210750.07.327509145
TGCCGTC59800.07.2743526145
ACGATCT162500.07.2277966145
CGATCTC180650.07.0233507145
GCGTATG21752.6131602E-87.000102145
TCGCGTA20750.06.9880533145
Produced by FastQC (version 0.11.5)