FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n01_Emily_2_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n01_Emily_2_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10552708
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT4450.004753287411.4041986
TGTATCG81300.09.987819145
CGTATGC253950.09.7353145
TCGTATG223700.09.042353145
GCCGTCT189950.08.77873145
ATGCCGT216500.08.639826145
CCGTCTT184200.08.50173145
CGTCTTC173750.08.428878145
TATGCCG223650.08.428449145
CGATGTA457400.08.321591145
TCTCGTA251350.08.307241145
TATCTCG281550.08.214447145
CTCGTAT235400.08.100139145
CGGACGA11653.2254866E-58.090225145
TATCGCG64200.07.9050794145
GTATGCC264700.07.8608794145
CACCGAT549100.07.842928145
TGCCGTC203600.07.727253145
ACCGATG517900.07.6854563145
TCGCGTA57900.07.638261145