FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43801356
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2106730.4809736940564123No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA761930.17395123566494153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAG157150.038.546581
ATGCCGT28450.037.2177934
TCAGAGA170100.035.1645362
CAGACTC103900.032.7469671
CAGAGAG193150.031.0520883
GAGAGAA207800.028.6534945
ACAGACT80500.028.4607542
AGAACCC222300.027.7042398
GAACCCA231000.026.8100459
GTTCACT50850.026.5360181
TCACTAG50300.025.8086363
TTCACTA59700.023.6818432
CACTAGC60050.022.4636334
AGAATCC102350.021.6118533
GAATCCA104950.021.560174
ATTAGGC46450.019.8333458
CAACTGT48450.019.764871
AATCCAG120450.019.7490885
ACTAGCA71700.019.5217235
CTAGCAA71450.019.184026
Produced by FastQC (version 0.11.5)