FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43801356
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT785900.17942366898412915No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC438770.10017269784981087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAG168150.044.096951
TCAGAGA179950.041.9287032
ACAGACT72350.041.8343732
CAGACTC100650.037.8422931
CAGAGAG199500.037.4315873
GAGAGAA210950.035.603895
AGAACCC250500.030.4445158
GAACCCA262850.029.1241879
GTTCACT57050.028.9390771
AGAGCAC664900.026.9554028
TCACTAG61200.026.7213553
GAATCCA111750.026.5470314
AGAATCC109250.026.2286053
CAACTGT44000.025.3439041
CACTAGC64050.025.0791874
TTCACTA68750.024.7508832
ATTAGGC47750.023.941978
AACTGTG48100.023.63472
AATCCAG130950.023.3730035
TATTAGG50200.022.917577
Produced by FastQC (version 0.11.5)