FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l01_n01_Emily__1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l01_n01_Emily__1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8809711
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2742313.112826289080312No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1436261.6303145472081888TruSeq Adapter, Index 3 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1372851.55833715771153No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC449650.5104026681465488No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC305180.3464131797285972TruSeq Adapter, Index 3 (98% over 50bp)
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC285640.32423311048455505No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA277530.3150273601483635No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC203540.23104049610708No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT155120.17607842073366536No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT146900.16674780818576226No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC135080.1533307959818432No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC132500.15040220956169845No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG121090.13745059287415898No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA118750.1347944330977486No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC115170.13073073566204385No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT112970.12823349142781187No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA110040.12490761615222112No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC109650.12446492285615271No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT105620.11989042546344596No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA101910.11567916359571842No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG101290.11497539476607122No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG99460.11289814160759643No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA99180.11258031052323965No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC97690.11088899511005526No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC96370.10939064856951607No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC95240.10810797312193329No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG88380.10032111155519176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTCA110750.037.4737172
CAGACTC117500.035.691471
GACTCAG123200.034.4850543
ACAGACT84550.033.2102282
ACTCAGA154450.033.185884
TCAGAGA210200.032.8608442
CTCAGAG218250.032.4799081
GAGCACA858200.031.703849
AGAGCAC869500.031.291828
AGAACCC223250.030.2978658
AAGAGCA922950.029.5503397
AGAGAAC232850.029.391226
CGGAAGA930750.029.3416444
TCGGAAG931350.029.2308253
GAGAACC233350.028.9554257
ATCGGAA942400.028.8561782
GATCGGA955750.028.498661
GAAGAGC969000.028.1684576
GAACCCA245850.028.0140089
GAGAGAA246400.027.8043635
Produced by FastQC (version 0.11.5)