FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_nbdam_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_nbdam_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225768
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG862397.035507534867936TruSeq Adapter, Index 6 (100% over 63bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC335992.7410570352627905Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA314652.566962100495363Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA287382.3444893324022162Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCGCGGC147751.2053667578204033No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCG141151.1515229635624358No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGC125661.0251532100691159No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC110030.8976413154854753Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAATACGACTCACTATAGGGCAGCGTGGTCGCGG88300.7203647019664406No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT80840.6595048981536473No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG79510.6486545577956024No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCG78230.6382121249698148No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAG74560.6082717121021269No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC69460.5666651438118796No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC68760.5609544383602769No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATACGACTCACTATAGGGCAGCGTGGTCGCGGCC64640.5273428577022732No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGCTAATACGACTCACTATAGGGCAGCGTGGTCGCG57550.4695015696281841No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC49870.40684697267345854No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATACGACTCACTATAGGGCAGCGTGGTCG44180.3604270955025747No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG44050.35936653591870565No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT39360.3211048093929683No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA34130.27843768151885184No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGT32560.2656293850059718No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATACGACTCACTATAGGGCAGCGTGGTCG31090.2536369035576064No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCTATAGTGA28190.22997826668668134No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGT25190.20550381475124166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24790.20224055449318307No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC24690.2014247394286684No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACACG23220.18943225798030294No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCTATAGTGA22220.18127410733515642No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT22000.17947931419322416No Hit
GGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGC16470.13436474112556374TruSeq Adapter, Index 6 (100% over 60bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCG15250.12441179733848494No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTGGTCG15150.12359598227397028No Hit
GGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTC14060.11470359807076054No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCAGATCGGAAGAGCACACGT13560.11062452274818727No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC12760.10409800223207001No Hit
GGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTG12710.10369009469981269TruSeq Adapter, Index 6 (100% over 59bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTAC150.002355556269.00020640
ATGTAGA150.002356318568.99457666
AAAAAGG91700.067.1888369
TTGAAAA93700.067.0487661
TGCTTGA94100.066.9131358
CTGCTTG93950.066.909857
CTTGAAA94050.066.87260460
TGAAAAA94150.066.8382362
GCTTGAA94250.066.7700359
TCTGCTT94150.066.7676656
AAAAAAG92600.066.6475768
GAAAAAA94650.066.5580463
TTCTGCT94550.066.5216955
CTTCTGC98700.063.7919854
GTCTTCT100500.062.61255652
CCGTCTT100850.062.39016750
TCTTCTG100950.062.3701753
GCCGTCT101550.061.96010249
CGTCTTC101750.061.8044151
GTATGCC102750.061.24147845
Produced by FastQC (version 0.11.2)