FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_nbdam_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_nbdam_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90847100
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1511440.1663718489638084TruSeq Adapter, Index 5 (100% over 63bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT1488490.1638456263325962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG442550.045.4082222
CTAATAC464900.043.830291
TATGCCG268850.042.28184546
GTATGCC272950.041.6974245
ATAGGGC471150.040.42605216
CGTATGC285250.039.9359544
TACGACT503650.039.5224085
TATAGGG489400.038.99630415
CTATAGG498650.038.62666714
TAGGGCA506950.037.16288417
ATACGAC552100.036.266174
ACTATAG540500.035.8718413
CAGCGTG511550.035.10994322
CACTATA558250.035.01566312
ATGCCGT330100.034.75008447
GACTCAC575550.034.3589638
CTCGTAT329750.033.9924242
TCGTATG339550.033.6106643
AGCGTGG530900.033.52490623
AATACGA611700.033.1782653
Produced by FastQC (version 0.11.2)