FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_nbdam_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_nbdam_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885492
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG14752216.659890772587442TruSeq Adapter, Index 4 (100% over 63bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC53970.6094916724261766Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA39520.44630555668487126Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA35400.39977775067420146Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG32830.3707543377015264TruSeq Adapter, Index 4 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17090.1930000496898899No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCGCGGC15870.17922239839546827No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGC10410.11756176227453213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT301.13032E-769.0086442
GTTAACA150.002355252469.0008539
ATCTTAG150.002355779868.99695642
GTATGCC159200.067.306745
TCGTATG159400.067.243943
CTCGTAT155250.067.1525942
TCTCGTA154750.067.1281341
CGTATGC159850.067.0545944
GACCAAT159500.066.8848535
TTCTGCT160400.066.84994555
GCTTGAA160450.066.8253559
ACCAATC155200.066.8038936
CTTGAAA160500.066.78303560
GTCTTCT160600.066.766752
AAAAAGG157350.066.7243269
TGCTTGA160550.066.7192558
TGACCAA160200.066.63190534
CCAATCT155950.066.5894737
TTGAAAA160950.066.55720561
CTAATAC275750.066.534841
Produced by FastQC (version 0.11.2)