FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_nbdam_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_nbdam_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5045869
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2961185.868523340578204Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2912855.772742019263679TruSeq Adapter, Index 3 (100% over 63bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2817235.583240468589256Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2325164.608046701172781Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC764861.5158142234766696Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGC540161.0704994521260855No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCGCGGC378290.7497023803035711No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATACGACTCACTATAGGGCAGCGTGGTCG341710.6772074344379532No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAG315690.6256404991885441No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCG300170.5948826654041157No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG259350.5139848061850198No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCTATAGTGA220560.4371100399158202No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATACGACTCACTATAGGGCAGCGTGGTCG215090.4262694889621589No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC211380.41891693977786587No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC210070.4163207566427111No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCG207130.41049420823251653No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAATACGACTCACTATAGGGCAGCGTGGTCGCGG198560.3935100177987181No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA177400.35157472379881444No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG174990.34679853955780465No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT169890.3366912617033855No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTGGTCG154030.30525960939532915No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151500.30024560685186236No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCTATAGTGA147170.29166432977154183No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGCTAATACGACTCACTATAGGGCAGCGTGGTCGCG120380.23857139374803427No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC113480.22489684135676133No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATACGACTCACTATAGGGCAGCGTGGTCGCGGCC106330.21072683416870314No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCTATAGTGA98990.1961802813350882No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC78640.15585026087676868Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT77780.15414589637582743No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC73230.14512861907433586Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT69820.13837061564618502No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT62100.12307097152145646Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACACG53670.10636423577385777No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGT53120.10527423522093023No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCTATAGTGAG51800.10265823389390409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG314750.067.1943469
CTTGAAA322350.066.91324660
TGCTTGA322500.066.85068558
TTGAAAA322450.066.8289661
GCTTGAA323150.066.7582659
TGAAAAA324000.066.50925462
AAAAAAG318650.066.4254268
GAAAAAA325100.066.30543563
TCTGCTT325100.066.3041256
TTCTGCT325350.066.2624755
CTGCTTG325550.066.1925857
CTTCTGC336650.064.0690454
GTCTTCT342700.062.92790652
TCTTCTG344550.062.58001753
CCGTCTT346750.062.22337750
GCCGTCT347500.062.06861549
CGTCTTC349850.061.65229451
GTATGCC352800.061.18506245
ATGCCGT355100.060.77905347
TGCCGTC355250.060.75339548
Produced by FastQC (version 0.11.2)