FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_nbdam_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_nbdam_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435560
Sequences flagged as poor quality0
Sequence length75
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1683406.911757460296605TruSeq Adapter, Index 2 (100% over 63bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA136690.5612261656456831Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC130120.5342508499072082Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA110670.45439241899193616Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGC57180.23477146939512883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36560.15010921512916947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAC150.00235610868.9981956
CTAATAC858100.066.730961
TCTCGTA188950.066.4082441
CTCGTAT189700.066.3821142
TCGTATG191600.066.33469443
GTATGCC191500.066.3125745
TGTATCT189100.066.1352737
AAAAAGG186800.066.1225469
GTATCTC189550.066.01601438
ATGTATC190250.065.8248236
CTTGAAA192950.065.7825360
AAAAAAG188050.065.7013668
CGTATGC193700.065.68542544
TGCTTGA193000.065.5867358
GATGTAT193200.065.3375735
GCTTGAA194000.065.3375759
TTGAAAA194750.065.2099561
TTCTGCT194050.065.0678255
GAAAAAA194650.064.99531663
GTCTTCT194600.064.9902852
Produced by FastQC (version 0.11.2)