FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_nbdam_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_nbdam_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458381
Sequences flagged as poor quality0
Sequence length75
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG813865.580571880736241TruSeq Adapter, Index 1 (100% over 63bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC64730.4438483496425146Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA57320.39303858182463974Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA49810.3415431221333794Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGC49600.3401031691992696No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19480.1335727769355196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGA150.002356383768.9947665
CTAATAC537900.066.4269561
TCGTATG89450.066.2202643
TCTCGTA87600.066.1245141
ACGATCT87200.066.1113237
CACGATC87350.066.0767836
CGATCTC87350.066.0767838
CTCGTAT88250.066.0261442
GTATGCC90150.065.7443545
AAAAAAG88750.065.5420468
TTGAAAA90650.065.41753461
GCTTGAA90700.065.3814759
CTTGAAA90850.065.2735160
AAAAAGG89400.065.2584669
TGCTTGA91400.064.9562258
GTCTTCT91100.064.8337752
TAATACG549900.064.7156142
CGTATGC91900.064.5674944
TTCTGCT92000.064.42451555
GAAAAAA92200.064.28036563
Produced by FastQC (version 0.11.2)