FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3188705
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGG7350.024.87682749
CGCGGGG700.001332641624.6483251
CCTACTA7800.023.44309634
ACCTACT9000.021.08404233
CGCTATG11950.020.78763629
TACTCTA10050.020.6002924
GTACTCT10100.019.4733943
GCCGGGC1601.3604749E-619.4105551
CTATCTA9800.019.36198438
ACACCTA9450.019.35015931
CACCTAC9450.019.34955232
AGGACTG9100.018.95608762
GGCTGCC12750.018.9412243
CTTCCGA9300.018.5525021
AACGTCG13100.018.43544624
GACTATC9750.018.39888855
GGACTGT9700.018.13864963
GTCGCTA13550.018.07781427
CTAATGC10050.017.85022521
CTGCCAG6700.017.5102482
Produced by FastQC (version 0.11.2)