FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646234
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT23820.1446938892040864No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTC22770.13831569509559394No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC22730.13807271627241327No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT22730.13807271627241327No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG21900.13303090569141446No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC21820.13254494804505315No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG19680.11954558100488752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG8100.022.9990729
GGCTGCC8600.022.86604743
GTGTGCG7650.022.5501618
ACATCCG5650.021.98207755
CGCGGTT2057.2759576E-1121.8871351
AACGTCG8650.021.536724
GCCAGTA4550.021.2395861
CGGCCAG2301.4551915E-1120.9991515
CGGCAAT9600.020.8524061
CGGGATT7450.020.8444142
GAGGCGT8700.020.62048153
GGGATTA8050.020.5755963
GACTGTC9250.020.517674
AGGCGTT8800.020.38615654
TGTGTGC8650.020.3408347
GTCCTAT6400.019.9451143
ACCCGCT7450.019.913769
GTCGCTA9400.019.81834827
GCTGCCA9750.019.81518444
GGCAGAC2800.019.7224731
Produced by FastQC (version 0.11.2)