FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330390
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT64110.19249997748011494No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT52980.15908046805329104No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC51420.15439633196112168No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC46110.13845225333969896No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG41710.1252405874387084No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGA40190.12067655740018438No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTC39850.11965565594419872No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG38680.11614255387507168No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATT37690.11316992904734881No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG36700.11019730421962592No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCG34030.10218022513879756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCA6100.033.9446561
CTGCCAG6600.030.8488162
CTTCCGA7950.026.0455881
CCGGCAT2550.025.7136331
CCGCGGG951.049093E-525.4287191
CGCTATG20600.022.441129
AACGTCG20850.022.33882524
GCCAGTA11450.022.3036651
GGCTGCC20650.022.21969843
GGGATTA15800.022.0591223
TGCCAGT9400.021.6594833
CGCGGGG800.00289550621.5690021
ACATCCG11900.021.45283955
CGCGGTT3550.021.3867261
CAACGTC22300.021.35011923
ACCCGCT14800.021.2122259
CGGGATT16500.021.1235962
AACGCTT22400.020.94613636
GTCGCTA22250.020.93229327
TTCCGAT9800.020.7757342
Produced by FastQC (version 0.11.2)