FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4692075
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC75530.16097355647554654No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT71520.152427231022522No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTC63940.13627233153775248No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC60060.12800306900465147No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT59670.127171880244881No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATT59660.12715056771257918No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGA58550.12478487662707864No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG56380.12016005711758657No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG52350.11157110659995845No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG48550.10347234432527186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG1550.035.6215861
CTGCCAG11650.028.1369062
CCTGCCA11550.028.0847661
CGCGGTT5050.026.650061
GCCGGGC2350.024.963531
TGCCAGT14300.022.6811893
CCGGCAT3600.021.0884721
ACATCCG16900.021.02592755
AACGTCG29800.020.95424
GAGGCGT21700.020.82693953
TGACTTA21600.020.76275446
AGGCGTT21650.020.71546454
GCCCGCT1004.3304203E-420.7050461
GACTGTC32650.020.3963664
GCCAGTA16100.020.3599364
CGCTATG30350.020.34693329
CCTACTA4100.020.19530934
GCACACC20600.020.09791669
GGCTGCC30900.020.09684643
CGTTCAG22350.019.91251257
Produced by FastQC (version 0.11.2)