FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817434
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT45410.16117502663771363No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTC42120.14949773446334502No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC41490.1472616572384659No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC39570.14044694569597727No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG37040.13146714350717711No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG36380.12912458641444663No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT35590.1263206165610268No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG32170.11418191162596888No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATT29910.10616042824783119No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAG29750.10559253561929047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG450.00461915430.6729951
CCTGCCA7300.027.4166161
CTGCCAG7450.026.3990782
CCGGCAT3100.023.3757881
TGCCAGT8850.022.222953
AGGCGTT13650.021.98808354
GAGGCGT14050.021.11654753
CGCGGTT2950.021.0551911
GCCAGTA9700.020.6309244
GCGGAAT3050.020.3648591
CGCTATG15400.020.16220329
CGTTCAG15300.019.61716757
TGACTTA15250.019.22869746
GTGTGCG15950.019.035038
GCGCCGT2204.1836756E-918.8220651
ACGCTAG1651.8294613E-618.81905723
ACATCCG9800.018.65737555
AACGTCG16850.018.63258424
CGGGATT13200.018.5592
TAGTCAT11750.018.4984059
Produced by FastQC (version 0.11.2)