FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668257
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC29450.1103716770910748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA9850.029.77207634
TACTCTA11500.027.9030864
CTGTAGG10450.027.40083349
CACCTAC10850.027.027632
ACCTACT11150.026.30089233
ACACCTA11650.026.06003831
CGCGGGG809.5519856E-525.880771
GTACTCT12500.025.3948083
TGGACAC12200.024.88426628
TCTAGTT13250.024.7348737
AGGACTA11900.024.64188653
CTAATGC12300.024.4014821
GACTATC11800.024.26599355
GGACTGT10950.024.25977363
CTATCTA12100.024.236438
GTTACCT13300.023.34582114
TCTAATG13750.022.07954420
TAGTTGT14600.021.9751649
CTGCCAG6050.021.6722222
TCTAACC13600.021.56286441
Produced by FastQC (version 0.11.2)