FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412224
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC63670.2639472951102385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT17550.030.4700857
GTACTCT17500.030.3662933
TACTCTA17650.030.1082254
CCTACTA17350.028.43633734
ACCTACT17750.028.18426733
CACCTAC18050.028.09811832
CTGTAGG17550.027.91337449
ACACCTA18000.027.21835931
GGACTGT18200.026.34780963
CTATCTA18800.026.0585138
TAGTTGT20500.025.7487839
GACTATC19250.025.44724355
CTAATGC20500.025.07613221
AGGACTG18750.025.02348362
TGGACAC20050.024.60649528
AGGACTA19650.024.4035753
ATCTAAC20650.024.2251840
GTTACCT21250.024.18858114
TATCAGG20100.024.0293558
CAGGACT19900.023.92412661
Produced by FastQC (version 0.11.2)