FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2994019
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGTC13400.016.2211514
CTGTAGG4300.016.04589549
CGCTATG10900.015.50961329
CGGCAAT10950.015.1254841
GCCGGAT3756.730261E-1114.7221381
CCGGCAT3605.7843863E-1014.3770881
CTATAGG2404.007459E-614.37516714
AACGTCG12050.014.3152824
CCTACTA4850.014.22696934
GGCTGCC11900.014.20557243
GGCGCGC4700.013.94697168
GTGTGCG10200.013.8675778
CGTACTG13950.013.84916122
GCCGGGC1500.00652577813.8020051
GCCAGTA7550.013.7106011
CCTGCCA7300.013.7074711
CGCGTAT3801.3005774E-913.62039851
TCCCTAC3801.3042154E-913.6183519
CCGACAT13250.013.541591
ACATCCG8450.013.4730855
Produced by FastQC (version 0.11.2)