FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5505012
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC70250.1276109843175637No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT65450.11889165727522483No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT63050.1145319937540554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58370.10603064988777498No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTC57600.10463192450806648No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC56580.10277906751156944No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG55410.10065373154499936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG1750.029.5777951
AACGTCG26650.020.06506324
CGCTATG26400.019.99426329
AGGCGTT22750.019.56151854
GAGGCGT22600.019.53923653
GTGTGCG21350.019.0678638
GACTGTC30400.018.8409884
GGCGGGC24550.018.69020713
GCCAGTA17200.018.457461
GTCGCTA29100.018.37623827
CGTTCAG25100.018.28049357
GGCTGCC28950.018.2334443
CGGCAAT28050.018.0841061
TAGCCCG25300.017.86358634
GTGCGAG22800.017.85553710
CCTGCCA12200.017.819411
CAACGTC30150.017.62136323
CGGGCGC25650.017.61951415
TGACTTA26050.017.61399546
GCAATCG30300.017.3089283
Produced by FastQC (version 0.11.2)