FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7121752
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT87110.12231540778168068No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT83590.11737280377075753No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC80970.11369393373989996No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC76420.10730505639623508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA14800.029.37152134
ACCTACT16750.024.92230233
TACTCTA18700.024.7258324
GGACTGT17550.024.17940363
CGCGGGG2001.8189894E-1224.156361
CTGTAGG17450.024.11941149
CTAATGC18250.024.00813721
CTGCCAG16700.023.5551192
CACCTAC17850.023.38614532
ACACCTA18400.022.8749231
GACTATC18700.022.32282455
GTACTCT20850.022.1761673
AGGACTA19100.021.85548653
CCTGCCA18250.021.7454551
TGGACAC20400.021.13918728
TCTAGTT21900.021.109227
CTATCTA20450.021.0876538
AACGTCG34650.020.61046424
GTTACCT21550.020.49180814
CGCTATG34350.020.38814529
Produced by FastQC (version 0.11.2)