FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458021
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC36180.1046263166128835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCAG6400.025.3404162
CGCGGGG1151.6945487E-624.0077111
GCGGGCC1202.4533547E-623.0073931
CCTGCCA7800.021.237591
GAGGCGT13900.020.10438253
CGCTATG16950.019.74314129
GGGGCCC1056.013318E-419.7206231
AGGCGTT14400.019.64589554
CGTTCAG14400.019.4060357
TGCCAGT8600.019.258383
AACGTCG18100.018.48900824
GTCGCTA18500.018.08898427
CGGCAAT18150.018.0636561
CCGGCAT4400.018.0398861
CGCCTAT1351.5742202E-417.8946381
GGCTGCC18650.017.75825743
TAGCCCG15700.017.57967434
GGCGGGC15750.017.52335713
CCAGTTA19100.017.52099855
GCGGGTT3550.017.4985791
Produced by FastQC (version 0.11.2)