FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914269
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT34100.11701047501105767No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT31620.10850062228298074No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC30830.10578982242202076No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC30410.10434863768581419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGC1601.8189894E-1228.0395071
CCCCCCT800.002895536821.5688511
CGCTATG14850.020.21217729
GGCTGCC15200.020.2000243
CTTCCGA9100.019.720091
AACGTCG15100.019.42025224
GACTGTC14150.019.0203324
CCTGCCA6050.018.8237231
GTCGCTA16000.018.75910627
TACGTGG12450.018.56536155
CCTACTA3550.018.4648434
CTACGTG12850.017.98714354
GCACACC7950.017.7931169
GCGGGAC1753.2022108E-617.7480811
GCTACGT13100.017.64478353
TCGAAGG17800.017.6364787
CCGACAT17050.017.6092911
CAACGTC16900.017.5556623
AACGCTT16950.017.50417336
GCTGCCA18000.017.24945644
Produced by FastQC (version 0.11.2)