FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10633904
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT952730.8959362431708994No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA538700.5065872326851926No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108230.10177823685449858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA19350.024.788561
TTCCGAT19600.023.9416082
CCGATCT20550.022.8342824
CGCGGGG9150.020.3652691
CGGGGAT10800.018.8514921
CGCGAAT3250.018.0502431
TCCGATC26850.017.47663
GCGGAAT5950.017.39891
CGATCTA5250.016.4302855
CCGGGAT12400.016.1407531
GACTATC28850.014.70836555
CGGCAAT9850.014.0133271
GTCGCTA7700.013.88949227
GTACTCT35650.013.5497373
GCCGGGT37200.013.5433911
CGGGCAT28400.013.4872111
GCGGAGT12250.013.2397341
GCGGGAG28150.013.1166471
TCGCCGA4751.6370905E-1113.07357240
GCGGGGT22000.013.0188581
Produced by FastQC (version 0.11.2)