FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4126480
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66710.1616632093212617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG1359.094947E-1230.6744231
TACTCTA14650.021.6683834
CTGTAGG12750.020.83425149
ACCTACT13350.020.41619333
CCTACTA13250.020.30989534
CACCTAC13650.020.21974832
ACACCTA13750.020.07293931
GCGGGCC2002.8774593E-818.97981
GACTATC14600.018.66709955
GGACTGT13750.018.56611863
CTAATGC16350.018.35754621
CTATCTA15550.017.97190538
TCTAGTT18150.017.867317
GTACTCT17200.017.2524813
CGCTATG12000.016.96247329
GCGCACC7650.016.68523666
AGGACTA16400.016.40771753
TCTAACC19100.016.07686641
GTTACCT18250.016.06866314
TAGTTGT19500.015.74651059
Produced by FastQC (version 0.11.2)