FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269781
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA6350.021.73207734
CGGGCGC5550.020.51241315
ACACCTA7600.019.5186931
CACCTAC7250.019.50929832
ACCTACT7300.019.37652833
CTGTAGG6850.019.1376649
GGCGGGC5950.019.13595813
CGCGGCC2556.730261E-1118.9401631
TAGCCCG6250.018.21582634
CGCTATG6250.018.2146229
GGCCGCG1903.4934783E-718.1617226
CTAATGC8450.017.55488421
TACTCTA8850.017.546074
CGGCAAT7800.017.2551571
GACTATC8400.016.83801355
GTACTCT8950.016.5789133
GGACTGT8050.016.28591563
GCGGGCG7000.016.26592314
GTTACCT9250.016.0394514
CGTTCAG7550.015.99322457
Produced by FastQC (version 0.11.2)