FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481900
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34850.14041661630202668No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25610.10318707441879206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC1103.265837E-521.9571933
CGGGCGC5250.017.74320815
GGCGGGC5250.017.74320813
GTGTGCG4400.017.2493028
CTTCCGA10250.017.1689621
CGGCAAT8550.015.7396911
CGCGTAT2654.1680323E-815.625471
TAGCCCG6350.015.21228834
GCGGGCG6550.014.74838414
CGCGAAT1901.2806035E-414.5289451
CCGCCCT1901.2806035E-414.5289451
CCGATCT11650.014.512484
GTCCCCC2152.2649532E-514.44185769
CCGACAT8850.014.4263411
GCAATCG9100.014.408333
GGCGCAT7000.014.29256517
CGCGGCC1450.005217152614.27585731
GGGGCGA3454.9058144E-913.99999764
GTGCGAG5700.013.92104910
CCGCAAT3001.9036634E-713.8024981
Produced by FastQC (version 0.11.2)