FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256307
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTAT1253.4889708E-622.090691
GCCGGGC850.004108857420.3039461
CCCTCGG900.00573581719.16602511
GCGCACC4950.016.72671366
GGCTATC1452.7167547E-416.659922
CGCACCG5650.016.48617667
GGCGAGT1906.987806E-616.350021
CGCGTAT1704.940955E-516.2431561
CGCCGGG5100.016.23604458
GGCGCAC5150.016.07713165
GCGGTGT3805.456968E-1215.4416851
CTACCGC1350.00324399115.332828
CCTACTA1605.7583157E-415.09384533
AGGGCCG5550.014.91901453
TCGCGTA1400.004123301714.7899292
GTTGCGC1657.2696496E-414.63703859
GTCCCCC2404.004869E-614.37451969
GGCGCGC2657.094386E-714.32084668
CGTACTG5850.014.15337322
CGGCAAT6000.013.8066821
Produced by FastQC (version 0.11.2)