FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151417
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT28340.13172713611540673No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT26120.12140835551638757No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC26020.12094354557949483No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC23520.10932329715717594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG655.444126E-737.1625981
CTTCCGA7650.032.0271451
TTCCGAT8600.028.086052
CCGATCT9300.026.3412364
CCCCCCT809.5503296E-525.8810941
CGCGGTT1651.1459633E-1025.0968171
GCCGGGC1101.1511966E-625.0968171
CTGTAGG2850.021.7880249
CGGCAAT11400.021.1891981
CCTGCCA5900.021.0558051
TGACTTA10600.020.82976746
GAGGCGT10450.020.79862453
CCGGCAT2850.020.5837921
AGGCGTT10750.020.5391254
CTGCCAG5950.020.2974832
TAGCCCG11300.019.84611334
GGCGGGC11350.019.75685113
CGTTCAG11000.019.44596557
AGAGGCG11200.019.40585752
GCAATCG11900.019.1367363
Produced by FastQC (version 0.11.2)