FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543165
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT33320.13101784587315413No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT30130.11847442065300522No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28410.11171119451549545No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGA25800.10144839206264636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG400.002593297734.5098341
GCCGGGC700.001332077224.6498811
CGCTATG12500.023.18422529
CGCGGTT2700.023.0065561
AACGTCG13600.022.06963324
GGCTGCC13400.021.8841143
GCCAGTA6650.021.7956851
GTCGCTA13350.021.7072227
CCCTGTG13750.021.57711863
CTTCCGA7500.020.70591
GAACGCT14600.020.32131635
TATCCCT14300.020.26513560
CCGACAT14300.019.7888571
GACTGTC12950.019.7168054
CCAGTTA15000.019.54903655
CAACGTC15100.019.41995423
AACGCTT15850.019.1547636
CACGCTT15900.018.4486541
GTGTGCG8650.018.3484428
GCTGCCA16300.018.2015644
Produced by FastQC (version 0.11.2)