FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783999
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62540.22464088528767434No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTC42730.15348425053313597No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG37250.13380033541678715No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC37210.13365665720425907No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG35940.12909487395649208No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT32270.1159123979570395No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT30950.11117101694361241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG1100.037.6445351
CTTCCGA6400.028.0373361
CTGTAGG6550.026.86215649
GGACTGT6450.026.20840363
ACCTACT7000.025.13620433
CCTACTA7150.025.09139634
CACCTAC7100.024.7817332
GACTATC7650.024.35210455
ACACCTA7200.023.9592331
TACTCTA8050.023.5731584
CCGCGGG750.001991215423.0049931
AGGACTA8100.022.99921253
GTACTCT8300.022.0313423
TTCCGAT8150.022.0146772
CTAATGC8100.021.72186921
AGGACTG8150.021.58821562
CCGATCT8650.021.5391544
CGGCAAT14600.021.271741
CTATCTA8700.021.01727538
GTTACCT9150.020.73811114
Produced by FastQC (version 0.11.2)