FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089054
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140090.6705906118271715No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60400.28912608290642555No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT32960.15777476312244682No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT24320.11641633007093163No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC23540.11268258264266985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22540.10789572696541112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG252.5643388E-455.214521
CTGTAGG5150.028.13523949
CCTACTA5900.026.3135534
AGGACTA6400.025.33532153
CTAATGC6650.024.90284221
GTTACCT6700.024.71759214
GACTATC6100.024.31849955
CCGGGAT1803.4197E-1023.006051
CACCTAC6450.022.99944132
GGACTGT6000.022.99889263
TCTGTAG6500.022.82252148
ACCTACT6700.022.6572533
TCTAATG7050.022.5111720
ACACCTA6800.022.32245331
ATCTAAC6900.021.99999240
GCGCGGT800.002895795321.568171
GTACTCT7600.021.337563
TACTCTA7600.021.337564
CCCGCTT1304.9137907E-621.2363551
TCAGGAC6700.021.11092260
Produced by FastQC (version 0.11.2)