FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14344133
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1111500.7748812702726613No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524810.36587084071236653No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC343090.23918489880148214No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCA159770.11138351826492407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155600.10847640634676212No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC146990.10247395224235582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA100750.030.0697254
GTACTCT100650.029.9280873
CCTACTA101650.027.79629334
CTGTAGG100150.027.42012449
ACCTACT105150.026.90406833
GGACTGT102000.026.4832963
CACCTAC107300.026.4613532
ACACCTA107750.026.3510231
TCTAGTT116050.026.01177
GACTATC106250.026.00814455
AGGACTA109450.025.34239253
GTTACCT116000.025.28019314
CTAATGC114550.025.26863321
CTATCTA112800.025.04913538
TATGCCG16250.024.83923748
TAGTTGT120850.024.7794959
TGGACAC116200.024.70208428
AGGACTG110300.024.5529162
TCTGTAG114500.024.16432648
ATCTAAC117900.023.99492840
Produced by FastQC (version 0.11.2)